Automated method for determination of serum 5'-nucleotidase activity.

Simple, direct determination of serum 5'-nucleotidase.

A simple determination of 5'-Nucleotidase in blood serum without deproteinization is described. The enzyme activity is distinguished from that of a nonspecific alkaline phosphatase by nickel inhibition and the inorganic phosphate released from adenosine monophosphate used as substrate is determined by a method previously described. Additional studies include the determination of optimal conditi...

Determination of serum 5'-nucleotidase with a centrifugal analyzer.

We describe a multi-point method for the determination of the serum enzyme activity 5'-nucleotidase by means of an NADH sensor reaction. Studies show that the assay may be carried out in one step, without preincubation, by using a GEMSAEC Fast Analyzer.

Routine determination of 5' nucleotidase activity of human serum using the LKB 8600 reaction rate analyser.

Inhibition by concanavalin A as the basis for a specific assay of serum 5'-nucleotidase activity.

Concanavalin A inhibits serum 5'-nucleotidase activity, without causing significant inhibition of alkaline phosphatase activity. This observation serves as the basis for a new method for assaying the 5'-nucleotidase activity in serum, which depends upon the difference between the enzymic hydrolysis of adenosine-5'-monophosphate in the presence and absence of concanavalin A. A denosine released ...

Colorimetric determination of serum 5'-nucleotidase without deproteinization.

We present a sensitive colorimetric determination of 5'-nucleotidase based on the measurement of liberated phosphate by reaction with stannous chloride/molybdenum blue. No protein precipitation is required. The method is simple, sensitive, economical, and easy to use, and involves relatively stable reagents.